The revolutionary ImageStream®X Mk II Imaging Flow Cytometer combines the speed, sensitivity, and phenotyping abilities of flow cytometry with the detailed imagery and functional insights of microscopy. This unique combination enables a broad range of applications that would be impossible using either technique alone.
By collecting large numbers of digital images per sample and providing a numerical representation of image-based features, the ImageStreamX Mk II combines the per cell information content provided by standard microscopy with the statistical significance afforded by large sample sizes common to standard flow cytometry. With the ImageStreamX Mk II System, fluorescence intensity measurements are acquired as with a conventional flow cytometer; however, the best applications for the ImageStreamX Mk II take advantage of the system’s imaging abilities to locate and quantitate the distribution of signals on in or between cells.
The instrument in the Core lab is equipped with the 405, 488, 642 and 785 lasers and can analyze five colors plus Brightfield at one time.
| Laser | 375 nm | 405 nm | 488 nm | 561 nm | 592 nm | 642 nm |
|---|---|---|---|---|---|---|
| Example Dyes | DAPI Hoechst 33258 Alexa Fluor 405 Marina Blue Pacific Blue Cascade Blue LIVE/DEAD Violet DyLight 405 eFluor 450 Spectrum Aqua DyeCycle Violet Alexa Fluor 430 Pacific Orange Cascade Yellow Lucifer Yellow Qdot 525 Qdot 545 Qdot 565 Qdot 585 Qdot 605 Qdot 625 EFluor 605 Qdot 705 eFluor 650 Qdot 800 | CFP DAPI Hoechst 33258 Alexa Fluor 405 Marina Blue Pacific Blue Cascade Blue LIVE/DEAD Violet DyLight 405 eFluor 450 Spectrum Aqua DyeCycle Violet Alexa Fluor 430 Pacific Orange Cascade Yellow Lucifer Yellow Qdot 525 Qdot 545 Qdot 565 Qdot 585 Qdot 605 Qdot 625 EFluor 605 Qdot 705 eFluor 650 Qdot 800 | FITC GFP YFP Acridine Orange Alexa Fluor 488 Alexa Fluor 599 Alexa Fluor 514 SYTO Spectrum Green LysoTracker Green DyeCycle Green Calcium Green-1 MitoTracker Green LysoTracker Red RFP mCherry Alexa Fluor 568 Alexa Fluor 594 Alexa Fluor 610 DyLight 594 Texas Red PE-Alexa Fluor 647 PE-Alexa Fluor 680 PE-Cy5 PE-Cy5-5 DRAQ5 Nile Blue PC-Cy7 PE-Alexa Fluor 750 | DsRed Dil Cy3 R-phycoerythrin OFP Alexa Fluor 546 Alexa Fluor 555 DyLight 549 Calcium Orange PE-Texas Red (ECD) PE-Alexa Fluor 610 Propidium Iodide Spectrum Orange MitoTracker Red LysoTracker Red RFP mCherry Alexa Fluor 568 Alexa Fluor 594 Alexa Fluor 610 DyLight 594 Texas Red PE-Alexa Fluor 647 PE-Alexa Fluor 680 PE-Cy5 PE-Cy5.5 DRAQS Nile Blue PE-Cy7 PE-Alexa Fluor 750 | mCherry Alexa Fluor 568 Alexa Fluor 594 Alexa Fluor 610 DyLight 594 Texas Red Spectrum Red Calcium Crimson Nile Blue APC APC-Cy5.5 DyLight 649 MitoTracker Deep Red APC-Cy7 APC-Alexa Fluor 750 APC-eFluor 780 | Nile Blue APC APC-Cy5.5 DyLight 649 MitoTracker Deep Red Alexa Fluor 647 Alexa Fluor 660 Alexa Fluor 680 DRAQS Cy5 Cy5.5 APC-Cy7 APC-Alexa Fluor 750 APC-eFluor780 DyLight 750 |
More information on the instrument can be found here:
https://www.luminexcorp.com/imagestreamx-mk-ii/