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Department of Microbiology and Immunology: Manzano Lab

Publications

In Revision

  1. Viswanathan P, Bersonda JR, Gill J, Navarro A, Farrar AC, Dunham D, Boehme KW, Mark Manzano. The Mitochondrial Ubiquitin Ligase MARCHF5 Cooperates with MCL1 to Inhibit Apoptosis in KSHV-Transformed Primary Effusion Lymphoma Cell Lines. bioRxiv. In revision for PLOS Pathogens.
  2. Maddineni A, Liang Z, Jambardi S, Roy S, Tycko J, Patil A, Manzano M, Gottwein E. Cytotoxicity of Activator Expression in CRISPR-based Transcriptional Activation Systems. bioRxiv. In revision for Nature Communications.

Publications

Google Scholar link

At UAMS

  1. Owens SM, Sifford JM, Li G, Murdock S, Salinas E, Oldenburg D, Ghosh D, Stumhofer J, Nookaew I, Manzano M, Forrest JC. Intrinsic p53 Activation Restricts Gammaherpesvirus-Driven Germinal Center B Cell Expansion during Latency Establishment. Nature Communications, 2025.
  2. Murdock SJ, Bersonda JR, Forrest JC, and Manzano M (Corresponding Author). Molecular Mechanisms of KSHV Latency Establishment and Maintenance. Current Clinical Microbiology Reports, 2024. PDF.
  3. Kuehnle N, Osborne SM, Liang Z, Manzano M, and Gottwein E. CRISPR screens identify novel regulators of cFLIP dependency and ligand-independent TRAIL-R1-mediated cell death. Cell Death and Differentiation. 2023.
  4. Dunham D*, Viswanathan P*, Gill J, Manzano M (Corresponding Author). Expression ratios of the antiapoptotic BCL2 family members dictate the selective addiction of Kaposi’s sarcoma-associated herpesvirus-transformed primary effusion lymphoma cell lines to MCL1. Journal of Virology. 96(23):e0136022, 2022. *Equal contributions.

Prior to UAMS

  1. Manzano M*, Gunther T*, Ju H, Nicholas J, Bartom ET, Grundhoff A, Gottwein E. KSHV drives a super-enhancer-mediated survival gene expression program in primary effusion lymphoma. mBio 11(4):e01457-20. 2020. (*Equal contributions.)
  2. Morrison K, Manzano M, Chung K, Schipma MJ, Bartom ET, Gottwein E. The oncogenic Kaposi’s sarcoma-associated herpesvirus encodes a mimic of the tumor suppressive miR-15/16 family. Cell Reports 29(10):2961-2969.e6. 2019.
  3. Patil A, Manzano M, Gottwein E. Genome-wide CRISPR screens reveal genetic mediators of cereblon modulator toxicity in primary effusion lymphoma. Blood Advances 3(14):2105-2117, 2019.
  4. Manzano M, Patil A, Waldrop A, Dave S, Behdad A, and Gottwein E. Gene essentiality landscape and druggable oncogenic dependencies in herpesviral primary effusion lymphoma. Nature Communications, 9(1):3263, 2018.
  5. Patil A, Manzano M, and Gottwein E. CK1α and IRF4 are essential and independent effectors of immunomodulatory drugs in primary effusion lymphoma. Blood 132:577-86, 2018.
  6. Balasubramanian A*, Manzano M*, Teramoto T, Pilankatta R, and Padmanabhan R. High-throughput screening for the identification of small-molecule inhibitors of the flaviviral protease. Antiviral Research 134:6-16, 2016. (*Equal contributions)
  7. Manzano M, Forte E, Raja AN, Schipma MJ, and Gottwein E. Divergent target recognition by coexpressed 5′-isomiRs of miR-142-3p and selective viral mimicry. RNA 21(9):1606-1620, 2015.
  8. Forte E, Raja AN, Shamulailatpam P, Manzano M, Schipma MJ, Casey JL, and Gottwein E. MicroRNA-mediated transformation by the Kaposi’s sarcoma-associated herpesvirus Kaposin locus. Journal of Virology. 89(4):2333-2341, 2015.
  9. Grosswendt S*, Filipchyk A*, Manzano M, Klironomos F, Schilling M, Herzog M, Gottwein E, and Rajewsky N. Unambiguous identification of miRNA:target site interactions by different types of ligation reactions. Molecular Cell. 54(6):1042-1054, 2014. (*Equal contributions)
  10. Zhang W, Nandakumar N, Shi Y, Manzano M, Smith A, Graham G, Gupta S, Vietsch EE, Laughlin SZ, Wadhwa M, Chetram M, Joshi M, Wang F, Kallakury B, Toretsky J, Wellstein A, and Yi C. Downstream of mutant KRAS, the transcription regulator YAP is essential for neoplastic progression to pancreatic ductal adenocarcinoma. Science Signaling 7(324):ra42, 2014.
  11. Boonyasuppayakorn S, Reichert ED, Manzano M, Nagarajan K, Padmanabhan R. Amodiaquine, an antimalarial drug, inhibits dengue virus type 2 replication and infectivity. Antiviral Research 106:125-134, 2014.
  12. Manzano M, Padia J, and Padmanabhan R. Small molecule inhibitor discovery for dengue virus protease using high-throughput screening. Methods in Molecular Biology 1138:331-344, 2014.
  13. Manzano M and Padmanabhan R. Targeted mutagenesis of dengue virus type 2 replicon RNA by yeast in vivo recombination. Methods in Molecular Biology 1138:151-160, 2014.
  14. Manzano M, Shamulailatpam P, Raja AN, Gottwein E. Kaposi’s sarcoma-associated herpesvirus encodes a mimic of cellular miR-23. Journal of Virology 87(21):11821-11830, 2013.
  15. Teramoto T, Chiang HS, Takhampunya R, Manzano M, Padmanabhan R, and Maric M. Gamma interferon-inducible lysosomal thioreductase (GILT) ablation renders mouse fibroblasts sensitive to dengue virus replication. Virology 441(2):146-151, 2013.
  16. Manzano M, Reichert ED, Polo S, Falgout B, Kasprzak W, Shapiro BA, and Padmanabhan R. Identification of cis-acting elements in the 3′-untranslated region of the dengue virus type 2 RNA that modulate translation and replication. Journal of Biological Chemistry 286(25):22521-22534, 2011.
  17. Alcaraz-Estrada SL, Manzano MI, Del Angel RM, Levis R, and Padmanabhan R. Construction of a dengue virus type 4 reporter replicon and analysis of temperature-sensitive mutations in non-structural proteins 3 and 5. Journal of General Virology 91(Pt 11):2713-2718, 2010.