The BD FACS Symphony is a self-service instrument and you must be trained and approved by the core prior to use. Once trained, the instrument is available for use all hours. To inquire about training, please contact core personnel, Andrea Harris by email at aharris@uams.edu.
The BD FACS Symphony has four lasers and can detect 16 colors. The Symphony is located in BioMed 2 Room 319-2. After training is completed, you will be given badge access and the instrument is available all hours.
Use the chart below along with the BD Spectrum viewer to see if your panel is compatible.
| Excitation Laser | Detection Filter | Detector | Example |
|---|---|---|---|
| 488 nm (blue) | 488/10 (483-493 nm) | SSC | SSC ONLY |
| 695/40 (675-715 nm) | PMT A | PERCP, PERCP5.5, 7AAD, EF710 | |
| 515/20 (505-525 nm) | PMT B | AF488, GFP, FITC | |
| 561 nm (green) | 780/60 (750-810 nm) | PMT A | PE-CY7 |
| 710/50 (685-735 nm) | PMT B | PC CY5 | |
| 670/30 (655-685 nm) | PMT C | PE CY5.5 | |
| 610/20 (600-630 nm) | PMT D | PE TXRED, PI, MCHERRY, PE CF594,tdtomato | |
| 582/15 (574-590 nm) | PMT E | PE | |
| 633 nm (red) | 780/60 (750-810 nm) | PMT A | APC-Cy7, EF780 |
| 720/40 (700-740 nm) | PMT B | AF 700, RF710 | |
| 660/20 (650-670 nm) | PMT C | APC, AF 647, EF660 | |
| 407 nm (violet) | 780/60 (750-810 nm) | PMT A | BV785 |
| 710/50 (685-735 nm) | PMT B | BV 711 | |
| 660/20 (650-670 nm) | PMT C | BV650 | |
| 610/20 (600-630 nm) | PMT D | BV605 | |
| 525/50 (500-550 nm) | PMT E | BV510, AMCYAN,BV480 | |
| 450/50 (425-475 nm) | PMT F | DAPI, BV421, EF450, PACBLUE |