Second-year graduate student Amrita Khakurel (Cell Biology Track) is the first author of the article entitled “The Golgi-associated retrograde protein (GARP) complex plays an essential role in the maintenance of the Golgi glycosylation machinery” in the journal Molecular Biology of the Cell.
The super-resolution microscopy image from the paper taken at the UAMS Digital Microscopy Core was chosen as a cover image for the Journal issue. Amrita utilized the CRISPR/Cas9 knock-out approach to delete multiple subunits of evolutionarily conserved GARP complex in three different human cell lines to show significant defects in both N- and O-glycosylation, reduced stability of Golgi resident proteins, and mislocalization of key glycosylation enzymes. Significantly all these defects were rescued upon the expression of missing GARP subunits. The senior author of the study is Dr. Vladimir Lupashin (Physiology and Cell Biology) in collaboration with Tetyana Kudlyk, MS (Physiology and Cell Biology) and Dr. Juan S. Bonifacino (NICHD).
“The Golgi-associated retrograde protein (GARP) complex plays an essential role in the maintenance of the Golgi glycosylation machinery,” Amrita Khakurel, Tetyana Kudlyk, Juan S. Bonifacino, and Vladimir V. Lupashin. Molecular Biology of the Cell, Vol. 32, No. 17: 1594-1610.