Digital Microscopy Core

Zeiss AxioImager Microscope

• Upright microscope for slide imaging
• Wide field differential interference contrast (DIC), fluorescence, polarized light, dark-field imaging
• Zeiss MRC5 color camera for immunohistochemical (IHC) imaging
• Zeiss MRm monochrome camera for sensitive fluorescence imaging
• Objectives: 5x, 10x, 20x, 40x oil, 63x oil, 100x oil
• Fluorescent filters: DIC, DAPI, CFP, GFP, YFP, DsRed, TexRed, Cy5
• Zeiss Zen software

Zeiss AxioObserver Microscope

• Inverted microscope for cell cultures in dishes and multi-well plates
• DIC, phase contrast, and fluorescence imaging
• Zeiss ICm1 monochrome camera
• Heated stage insert for live cell time-lapse imaging
• Objectives: 2.5x, 10x, 20x, 40x oil, 63x oil
• Fluorescent filters: DAPI, GFP, DsRed, PI, Cy5
• Zeiss Zen software

Bruker Ultima In-vivo

• Multi Photon
• Mai-tai Deep Sea tunable laser
• Faraday cage for neurophysiology studies
• Pico-spritzer for localized liquid delivery
• Objectives: 4x, 16x water, 40x water
• Fluorescent filters: DAPI, GFP, DsRed, Cy5
• Fluorescent filters: DAPI, GFP, DsRed, Cy5

Zeiss LSM 880 Confocal Microscope with Airyscan:

• Laser scanning confocal light microscope for fluorescence and differential interference contrast microscopy
• Heated stage insert for live cell time-lapse imaging
• Objectives: 10x, 20x, 40x oil, 63x water, 63x oil, 100x oil
• Excitation lasers (nm): 405, 458, 488, 514, 561, 594, 633
• Controlled by Zeiss ZEN 2 software

Zeiss ELYRA S1 Super-Resolution Microscope

• Structured illumination microscopy (SIM) and localization microscopy (PALM/STORM)
• Heated stage insert for live cell imaging
• Objectives: 10x, 20x, 64x oil, 100x oil
• Controlled by Zeiss ZEN 2 software
• Excitation lasers (nm): 405, 488, 561, 642

FEI Tecnai F20 200 keV Transmission Electron Microscope (TEM)

• Electron microscope with field emission gun and 4 K FEI Eagle camera
• Tomography and Cryo-TEM available
• Controlled by FEI software with SerialEM software for tomography

Leica Ultracut 7 Microtome with FC7 Cryo-attachment:

• Electronic controlled microtome for sectioning plastic embedded and cryofrozen samples for electron microscopy
• Diatome diamond knives for ultra-thin plastic and cryo sections
• Glass knife for cutting tomography sections

Leica EMPACT2/AFS2

• Automated high pressure freezing and freeze substitution for electron microscope sample preparation

FEI Vitrobot

• Electronic controlled plunge freezing of samples for electron microscopy

HP Workstation PC for Image Processing and Analysis

• Full Zeiss ZEN software for confocal and super-resolution image processing
• FIJI software for analysis
• eTomo and 3dmod for TEM tomography processing
• MS Office

• Availability:
  • The Digital Microscopy Core is a shared research facility available to all investigators in the state of Arkansas.
  • The facility is accessible to trained users 24 hours a day, 7 days a week.
  • Training and technical assistance is available 9:00 a.m. – 5:00 p.m. Monday through Friday with prior scheduling.
  • Access to scheduling and sample submission through iLab core management
• Online ILab scheduling:
  • Trained users may reserve time using online scheduling.
  • Register for iLab account/Login:  https://uams.ilab.agilent.com/landing/1565
  • Training and technical assistance scheduled with facility approval.
• TEM Sample processing:
  • EM sample submission done through iLab request for services.
  • Limited to 48-hour prior notice with arranged exceptions
• Fees: View our Current Fees

• Acknowledgement: All users agree to acknowledge use of the Digital Microscopy Core in scholarly work and to provide a copy of published work.

Vladimir Lupashin, Ph.D. – Core Director
Department of Physiology & Cell Biology, College of Medicine vvlupashin@uams.edu
501-603-1170

Brian Storrie, Ph.D.
Department of Physiology & Cell Biology, College of Medicine StorrieBrian@uams.edu
501-526-7418

Jeff Kamykowski
Lab Manager Department of Physiology & Cell Biology, College of Medicine jakamykowski@uams.edu
501-526-7290

Biomedical Research Building II, Room 136-2
4301 West Markham Street, #505
Little Rock, AR 72205