The MiSeq is an integrated instrument that performs clonal amplification, genomic DNA sequencing, and data analysis with base calling, alignment, variant calling, and reporting in a single run. The MiSeq benchtop instrument utilizes a double-sided, single-lane flow cell and reagent cartridge supplied in kit form. Sequencing is performed by recording the synthesis of DNA strands in clusters of sample templates attached to the flow cell. Each newly attached base liberates a fluorescent dye that is excited by diode lasers (530 & 660 nm) and imaged using two digital cameras. Sequential interrogation of bases allows for the flexible adjustment of read length during a run. Up to 96 samples may be sequenced in a single run with DNA libraries prepared with indexed or bar-coded adapters. Key applications include small whole-genome sequencing (microbe/virus), targeted gene sequencing (amplicon, gene panel), and 16S metagenomic sequencing.
Fees for services vary greatly and are dependent on the service requested. If you are interested in MiSeq services, please contact Jennifer Johnson at johnsonjennifer@uams.edu.
For more information regarding the MiSeq capabilities, go to MiSeq Applications.