RNA Sequencing and Transcriptional Analysis for total RNA or mRNA
mRNA is fragmented, converted to cDNA, and barcoded. Maximum number of reads is 22-25 million.
Note: The Miseq is designed for organisms with smaller genomes and may not be suitable for RNA-seq if you are working with mouse or human. Please inquire if you are not sure of your requirements.
Library Generation: $470/library
300 cycle (2 X 150) – $1045/run
If you prefer a different number of cycles:
600 cycle (2 x 300) – $1540/run
500 cycle (2 x 250) – $1210/run
50 cycle (2 x 25) – $820/run
Mapping and Differential Expression: $200/sample
Custom analysis: $50/hour
RNA should be of very high quality with a RIN value of 8.0 or above.
If your RNA is polyadenylated (i.e. eukaryotic), we recommend Qiagen’s RNeasy kit.
Please provide at least 1ug of total RNA in less than 50ul.
If your RNA is non-polyadenylated (i.e. prokaryotic) you will need to do a depletion of your ribosomal RNA. We recommend Illumina’s Ribozero Kit. Please provide 5ul of pre-depleted sample when submitting sample for quality check and 100ng of depleted sample.
All requests must be submitted through iLab. See the links below for your particular institution.
Please provide a gel documentation image of your samples, concentration, and 260/280 ratios. This is for QC purposes and we will not sequence your samples without this information.
Bring or ship your samples to:
Data will be delivered via Basespace, Illumina’s cloud computing service. Each customer is allowed 1 free Tb of space, including a variety of free apps for further analysis.
For publication purposes, please cite the following:
The study was supported in part by the Center for Microbial Pathogenesis and Host Inflammatory Responses grant P20GM103625 through the NIH National Institute of General Medical Sciences Centers of Biomedical Research Excellence. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH.