Small genome sequencing using an Illumina Miseq. Genomes are enzymatically fragmented and “barcoded”. Instrument yields 22-25 million reads. Great for bacterial and viral genomes. Can also be used for PCR products or DNA fragments greater than 300 bp.
Library Generation: $330/library
600 cycle (2 x 300) – $1540/run
500 cycle (2 x 250) – $1210/run
300 cycle (2 X 150) – $1045/run
50 cycle (2 x 25) – $820/run
de novo assembly: $50/sample
Referenced assembly: $50/sample
Referenced alignment: $50/sample
Custom analysis: $50/hour
The method of DNA extraction will depend on your organism of study but genomic DNA should be very clean, free of RNA, and have a 260/280 of 1.8 – 2.0.
For each sample, please provide at least 100ng at a concentration that is at least 30ng/ul in 10 mM Tris-Cl, pH 8.5 (Qiagen’s EB Buffer). Please do not use TE!
All requests must be submitted through iLab. See the links below for your particular institution.
Please provide a gel documentation image of your samples, concentration, and 260/280 ratios. This is for QC purposes and we will not sequence your samples without this information.
Bring or ship your samples to:
UAMS DNA Sequencing Laboratory
Department of Microbiology and Immunology
UAMS, Biomedical Bld. II, Rm 321-2
4301 W. Markham
Little Rock, AR 72205-7199
Data will be delivered via Basespace, Illumina’s cloud computing service. Each customer is allowed one free Tb of space, including a variety of free apps for further analysis.
For publication purposes, please cite the following:
The study was supported in part by the Center for Microbial Pathogenesis and Host Inflammatory Responses grant P20GM103625 through the NIH National Institute of General Medical Sciences Centers of Biomedical Research Excellence. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH.