Overview of Services
The UAMS Experimental Pathology (ExPath) Core Laboratory provides researchers with centralized, comprehensive histological services. The laboratory can process and section tissues for use in biochemical, molecular, and pathologic studies. In addition, the laboratory works with investigators to develop immunostaining approaches to detect specific targets of interest and to identify clinical pathologists who can assist with image analysis and data interpretation. The laboratory director, consultants, and technicians have extensive experience in routine histology and immunohistochemistry involving human and animal tissues.
Services and Equipment
- Tissue Processing and Embedding: The laboratory can process and paraffin embed tissues for downstream analysis. The lab is equipped to section frozen and paraffin-embedded tissues. Sections can be prepared for slides, molecular and biochemical studies, and laser capture dissection.
- Tissue-Tek VIP 6 AI Tissue Processor and Tissue-Tek Embedding Center: For processing and paraffin embedding tissue.
- Microm HM325 Microtome: For sectioning paraffin-embedded tissues
- Leica CM3050S Cryostat: For sectioning frozen tissue.
- Tissue Staining: The laboratory provides a variety of standard pathological stains for the histochemical analysis of tissues. In addition, the laboratory works with investigators to develop immunostaining approaches for detecting specific targets of interest.
- Histo-Tek SL: Automated slide stainer for routine Hematoxylin and Eosin tissue staining.
- Leica Bond-Rx Fully Automated Research Stainer: Stainer capable of automated single and multiplexed immunostaining protocols.
- Slide Scanning and Imaging: The laboratory is equipped with an Aperio digital slide scanner that can capture whole-slide images at 20x and 40x for subsequent analysis and archiving. In addition, the laboratory has an Akoya Mantra™ multispectral imaging system for capturing images with up to seven different colors on a single slide.
- Leica (Aperio) Scanscope CS2: Has a five-slide capacity and can scan a whole slide at 20X or 40x magnification. Images can be stored on a server for sharing and remote analysis.
- Mantra™ Quantitative Pathology Workstation: Microscope equipped with a multispectral camera for multiplexed immunohistochemistry and imaging. inForm® Image Analysis software can spectrally unmix signals from nine different fluorophores.
- Consultation: Project planning is facilitated by consultation with investigators before project submission to the ExPath laboratory. ExPath staff can assist with antibody selection and optimization, protocol development, and troubleshooting. In addition, investigators can consult with clinical pathologists regarding data analysis and interpretation.
Visit the iLab Core Site for complete information.
Contact and Location
Director:
Steven Post, Ph.D.
Email: SPost@uams.edu
Lab Manager/Chief Technician:
Jennifer D. James, B.S., HT/ASCP, HTL, QIHC,
Email: JamesJenniferD@uams.edu
UAMS Cancer Institute, Room 429
Phone: 501-686-8265