The BD FACS Celesta is a self-service instrument and you must be trained and approved by the core prior to use. Once trained, the instrument is available for use all hours. To inquire about training, please contact core personnel, Andrea Harris aharris@uams.edu
Training links
VIDEO: Running 2 color flow on FACS Diva
VIDEO: 7 color compensation on FACSDiva
BD FACS Diva software tutorial
The BD FACS Celesta has 3 lasers and can detect 12 colors. The Celesta is also able to run samples from a 96 well plate.
Use the chart below along with the BD Spectrum viewer to see if your panel is compatible.
| Excitation Laser | Detection Filter | Example |
| 488 nm (Blue) | 780/60 (750-810 nm) | PECY7 |
| 695/40 (675-715 nm) | PERCP, PERCP5.5, 7AAD, EF710 | |
| 575/25 (562-588 nm) | PE | |
| 530/30 (545-515 nm) | FITC, GFP, AF488 | |
| 633 nm (red) | 780/60 (750-810 nm) | APC-Cy7, EF780 |
| 720/40 (700-740 nm) | AF 700 | |
| 660/20 (650-670 nm) | APC, AF 647, EF660 | |
| 407 nm (violet) | 780/60 (750-810 nm) | BV 785 |
| 660/20 (650-670 nm) | BV650 | |
| 610/20 (600-630 nm) | BV605 | |
| 525/50 (500-550 nm) | BV510, AMCYAN | |
| 450/50 (425-475 nm) | DAPI, BV421, EF450, PACBLUE |