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Department of Microbiology and Immunology: Flow Cytometry Core Facility
  • UAMS Health
  • Jobs
  • Giving
  • Training and Education
  • Protocols and Reagents
    • Immunofluorescence Staining for Immunotyping
    • Cell Cycle Staining using PI
    • Sorting and Staining Buffers
    • Lysing red blood cells
  • Sample Submission
    • Appointments and Pricing
    • Sample Preparation
  • Instruments
    • BD LSRFortessa
    • BD FACSAria IIIu
    • BD FACS Celesta
    • Cytek Northern Lights
    • ImageStreamX by Amnis
    • Hematology Analyzer-VETSCAN HM5
  • Data Analysis and Citation
  1. University of Arkansas for Medical Sciences
  2. College of Medicine
  3. Department of Microbiology and Immunology
  4. Research Cores
  5. Flow Cytometry Core Facility
  6. Sample Submission
  7. Sample Preparation

Sample Preparation

Best Practices for Flow Cytometry

Best-Practices-and-Instruction-for-Flow-CytometryDownload

Sample Prep Flow Cytometry (BD LSR Fortessa and BD Celesta)

FLOW PREPDownload

Sorting Prep (BD FACS AriaIII)

SORTING PREPDownload

Safety Guidelines

The UAMS flow core is a multi-user facility where many different samples from various sources that contain unknown animal and human pathogens are analyzed and sorted. The safety of the staff and users of the facility is the ultimate concern.

1. No samples BSL 3(or higher) or radioactive samples or any kind will be allowed in the core facility.

2. All samples for analysis must be fixed prior to being submitted for flow cytometry analysis. There are several fixation procedures available on-line and there is also one listed on the flow core website.

3. Unfixed samples may be submitted for sorting but must be BSL1 or BSL2.

Upon submitting samples you agree that these guidelines have been met and notify personnel of any safety concerns associated with the samples. Failure to follow these procedures will result in no longer being able to use the core facility.

UAMS College of Medicine LogoUAMS College of MedicineUniversity of Arkansas for Medical Sciences
Mailing Address: 4301 West Markham Street, Little Rock, AR 72205
Phone: (501) 686-7000
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