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Department of Microbiology and Immunology: Flow Cytometry Core Facility
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    • Immunofluorescence Staining for Immunotyping
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    • BD LSRFortessa
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  • Data Analysis and Citation
  1. University of Arkansas for Medical Sciences
  2. College of Medicine
  3. Department of Microbiology and Immunology
  4. Research Cores
  5. Flow Cytometry Core Facility
  6. Instruments
  7. BD LSRFortessa

BD LSRFortessa

Sample Prep and Information for BD LSR Fortessa

Best-Practices-and-Instruction-for-Flow-CytometryDownload
FLOW PREPDownload

The BD LSRFortessa has 16 detectors plus forward- and side-scatter detectors and an additional FSC PMT. The Fortessa is used for analysis only. The Fortessa can analyze up to 16 colors at one time. The Fortessa is also the instrument used for cell cycle, cell proliferation, cell membrane potential, and other analysis. To determine if your fluorochromes can be analyzed on the LSRFortessa, see the table below to determine which fluorochromes may be used based on your fluorochromes excitation and emission.

The BD Biosciences Fluorescence Spectrum Viewer will help you determine whether the fluorochromes you want to use will have a significant overlap.

BD LSRFortessa

Excitation LaserDetection FilterDetectorExample
488 nm (blue)488/10 (483-493 nm)SSCSSC ONLY
695/40 (675-715 nm)PMT APERCP, PERCP5.5, 7AAD, EF710
515/20 (505-525 nm)PMT BAF488, GFP, FITC
561 nm (green)780/60 (750-810 nm)PMT APE-CY7
710/50 (685-735 nm)PMT BPC CY5
670/30 (655-685 nm)PMT CPE CY5.5
610/20 (600-630 nm)PMT DPE TXRED, PI, MCHERRY, PE CF594
582/15 (574-590 nm)PMT EPE
633 nm (red)780/60 (750-810 nm)PMT A APC-Cy7, EF780
720/40 (700-740 nm)PMT BAF 700
660/20 (650-670 nm)PMT CAPC, AF 647, EF660
407 nm (violet)780/60 (750-810 nm)PMT ABV785, QD800
710/50 (685-735 nm)PMT BBV 711
660/20 (650-670 nm)PMT CBV650, QD655
610/20 (600-630 nm)PMT DBV605, QD605
525/50 (500-550 nm)PMT EBV510, AMCYAN
450/50 (425-475 nm)PMT FDAPI, BV421, EF450, PACBLUE

Analysis

The Fortessa computer uses FACS Diva software to collect data.  These files will be uploaded onto the Flow server for the user to then analyze in FlowJo or similar software. You are able to download FlowJo from their website and the core has a USB dongle you can check out free of charge.

Data Delivery

All data files are uploaded to a folder on a UAMS server under the Flow Core.

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Mailing Address: 4301 West Markham Street, Little Rock, AR 72205
Phone: (501) 686-7000
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