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Department of Microbiology and Immunology: Flow Cytometry Core Facility
  • UAMS Health
  • Jobs
  • Giving
  • Training and Education
  • Protocols and Reagents
    • Immunofluorescence Staining for Immunotyping
    • Cell Cycle Staining using PI
    • Sorting and Staining Buffers
    • Lysing red blood cells
  • Sample Submission
    • Appointments and Pricing
    • Sample Preparation
  • Instruments
    • BD LSRFortessa
    • BD FACSAria IIIu
    • BD FACS Celesta
    • Cytek Northern Lights
    • ImageStreamX by Amnis
    • Hematology Analyzer-VETSCAN HM5
  • Data Analysis and Citation
  1. University of Arkansas for Medical Sciences
  2. College of Medicine
  3. Department of Microbiology and Immunology
  4. Research Cores
  5. Flow Cytometry Core Facility
  6. Instruments
  7. BD FACS Celesta

BD FACS Celesta

The BD FACS Celesta is a self-service instrument and you must be trained and approved by the core prior to use. Once trained, the instrument is available for use all hours. To inquire about training, please contact core personnel, Andrea Harris aharris@uams.edu

Training links

VIDEO: Running 2 color flow on FACS Diva

VIDEO: 7 color compensation on FACSDiva

BD FACS Diva software tutorial

BD FACS Diva data tutorial-2

Best-Practices-and-Instruction-for-Flow-CytometryDownload

The BD FACS Celesta has 3 lasers and can detect 12 colors. The Celesta is also able to run samples from a 96 well plate.

Use the chart below along with the BD Spectrum viewer to see if your panel is compatible.

Excitation LaserDetection FilterExample
488 nm (Blue)780/60 (750-810 nm)PECY7
 695/40 (675-715 nm)PERCP, PERCP5.5, 7AAD, EF710
575/25 (562-588 nm)PE
530/30 (545-515 nm)FITC, GFP, AF488
633 nm (red)780/60 (750-810 nm)APC-Cy7, EF780
720/40 (700-740 nm)AF 700
660/20 (650-670 nm)APC, AF 647, EF660
407 nm (violet)780/60 (750-810 nm)BV 785
660/20 (650-670 nm)BV650
610/20 (600-630 nm)BV605
525/50 (500-550 nm)BV510, AMCYAN
450/50 (425-475 nm)DAPI, BV421, EF450, PACBLUE

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Mailing Address: 4301 West Markham Street, Little Rock, AR 72205
Phone: (501) 686-7000
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