The FACSAria has four lasers and is able to detect up to 15 colors at one time as well as sort up to four populations at once. The FACS Aria is used for analysis of more than four colors and cell sorting, its primary function being cell sorting.
Lasers
488-nm Coherent® SapphireTM solid state
633-nm JDS UniphaseTM HeNe air-cooled
407-nm Point Source Violet solid state
All lasers are Class IIIb.
BD FACS Aria IIIu, Cell Sorter
| Excitation Laser | Detection Filter | Detector | Example |
|---|---|---|---|
| 488 nm (blue) | 488/10 (483-493 nm) | SSC | SSC ONLY |
| 695/40 (675-715 nm) | PMT A | PERCP, PERCP5.5, 7AAD, EF710 | |
| 515/20 (505 – 525 nm) | PMT B | AF488, GFP, FITC | |
| 561 nm (green) | 780/60 (750- 810 nm) | PMT A | PE-CY7 |
| 710/50 (685-735 nm) | PMT B | PC CY5 | |
| 670/30 (655-685 nm) | PMT C | PE CY5.5 | |
| 610/20 (600-630 nm) | PMT D | PE TXRED, PI, MCHERRY, PE CF594 | |
| 582/15 (574-590 nm) | PMT E | PE | |
| 633 nm (red) | 780/60 (750-810 nm) | PMT A | APC-Cy7, EF780 |
| 720/40 (700-740 nm) | PMT B | AF 700 | |
| 660/20 (650-670 nm) | PMT C | APC, AF 647, EF660 | |
| 407 nm (violet) | 710/50 (685-735 nm) | PMT A | BV 711 |
| 660/20 (650-670 nm) | PMT B | BV650, QD655 | |
| 610/20 (600-630 nm) | PMT C | BV605, QD605 | |
| 525/50 (500-550 nm) | PMT D | BV510, AMCYAN | |
| 450/50 (425-475 nm) | PMT E | DAPI, BV421, EF450, PACBLUE |
Analysis
Analysis and acquisition on the FACS Aria is done in the FACS Diva software. Using FACS Diva, data may be analyzed on dot plots and histograms with various gates.
Data Delivery
Please bring a USB drive or blank CD to save your data files. The Core Facility does not store or archive data for longer than about six months so it is your responsibility to get the data files and save them for future use.