Sample prep and information for BD FACSAria
The FACSAria III has four lasers and is able to detect up to 15 colors at one time as well as sort up to four populations at once. The primary function of the FACS Aria is sterile cell sorting.
|Excitation Laser||Detection Filter||Example|
|488 nm (blue)||695/40 (675-715 nm)||PERCP/5.5, 7AAD, EPRCP-EF710|
|515/20 (505 – 525 nm)||AF488, GFP, FITC|
|561 nm (green)||780/60 (750- 810 nm)||PE-CY7|
|710/50 (685-735 nm)||PE-CY5|
|670/30 (655-685 nm)||PE-CY5.5|
|610/20 (600-630 nm)||PE-TXRED, PI, MCHERRY, CF594|
|582/15 (574-590 nm)||PE|
|633 nm (red)||780/60 (750-810 nm)||APC-Cy7, APC-EF780|
|720/40 (700-740 nm)||AF700|
|660/20 (650-670 nm)||APC, AF647, EF660|
|407 nm (violet)||710/50 (685-735 nm)||BV711|
|660/20 (650-670 nm)||BV650, QD655|
|610/20 (600-630 nm)||BV605, QD605|
|525/50 (500-550 nm)||BV510, AMCYAN|
|450/50 (425-475 nm)||DAPI, BV421, EF450, PACBLUE|
The Fortessa computer uses FACS Diva software to collect data. These files will be uploaded onto the Flow server for the user to then analyze in FlowJo or similar software. You are able to download FlowJo from their website and the core has a USB dongle you can check out free of charge.
All data files are uploaded to a folder on a UAMS server under the Flow Core.