• Skip to main content
  • Skip to main content
Choose which site to search.
University of Arkansas for Medical Sciences Logo University of Arkansas for Medical Sciences
Department of Microbiology and Immunology: Flow Cytometry Core Facility
  • UAMS Health
  • Jobs
  • Giving
  • Training and Education
  • Protocols and Reagents
    • Immunofluorescence Staining for Immunotyping
    • Cell Cycle Staining using PI
    • Sorting and Staining Buffers
    • Lysing red blood cells
  • Sample Submission
    • Appointments and Pricing
    • Sample Preparation
  • Instruments
    • BD LSRFortessa
    • BD FACSAria IIIu
    • BD FACS Celesta
    • Cytek Northern Lights
    • ImageStreamX by Amnis
    • Hematology Analyzer-VETSCAN HM5
  • Data Analysis and Citation
  1. University of Arkansas for Medical Sciences
  2. College of Medicine
  3. Department of Microbiology and Immunology
  4. Research Cores
  5. Flow Cytometry Core Facility
  6. Instruments
  7. BD FACSAria IIIu

BD FACSAria IIIu

Sample prep and information for BD FACSAria

Best-Practices-and-Instruction-for-Flow-CytometryDownload
SORTING PREPDownload

The FACSAria III has four lasers and is able to detect up to 15 colors at one time as well as sort up to four populations at once. The primary function of the FACS Aria is sterile cell sorting.

Excitation LaserDetection FilterExample
488 nm (blue)695/40 (675-715 nm)PERCP/5.5, 7AAD, EPRCP-EF710
515/20 (505 – 525 nm)AF488, GFP, FITC
561 nm (green)780/60 (750- 810 nm)PE-CY7
710/50 (685-735 nm)PE-CY5
670/30 (655-685 nm)PE-CY5.5
610/20 (600-630 nm)PE-TXRED, PI, MCHERRY, CF594
582/15 (574-590 nm)PE
633 nm (red)780/60 (750-810 nm)APC-Cy7, APC-EF780
720/40 (700-740 nm)AF700
660/20 (650-670 nm)APC, AF647, EF660
407 nm (violet)710/50 (685-735 nm)BV711
660/20 (650-670 nm)BV650, QD655
610/20 (600-630 nm)BV605, QD605
525/50 (500-550 nm)BV510, AMCYAN
450/50 (425-475 nm)DAPI, BV421, EF450, PACBLUE

Analysis

The Fortessa computer uses FACS Diva software to collect data.  These files will be uploaded onto the Flow server for the user to then analyze in FlowJo or similar software. You are able to download FlowJo from their website and the core has a USB dongle you can check out free of charge.

Data Delivery

All data files are uploaded to a folder on a UAMS server under the Flow Core.

UAMS College of Medicine LogoUAMS College of MedicineUniversity of Arkansas for Medical Sciences
Mailing Address: 4301 West Markham Street, Little Rock, AR 72205
Phone: (501) 686-7000
  • Facebook
  • Twitter
  • Instagram
  • YouTube
  • LinkedIn
  • Pinterest
  • Disclaimer
  • Terms of Use
  • Privacy Statement

© 2023 University of Arkansas for Medical Sciences