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  5. Protocol for the Analysis of Laser Capture Microdissected Fresh-Frozen Tissue Homogenates by Silver-Stained 1D SDS-PAGE

Protocol for the Analysis of Laser Capture Microdissected Fresh-Frozen Tissue Homogenates by Silver-Stained 1D SDS-PAGE

Methods Mol Biol. 2018;1723:95-110. doi: 10.1007/978-1-4939-7558-7_4.

Prieto DA, Whitely G, Johann DJ Jr, Blonder J

Abstract

The heterogeneity present in solid tumors adds significant difficulty to scientific analysis and improved understanding. Fundamentally, solid tumor formation consists of cancer cells proper along with stromal elements. The burgeoning malignant process is dependent upon modified stromal elements. Collectively, the stroma forms an essential microenvironment, which is indispensable for the survival and growth of the malignant neoplasm. This cellular heterogeneity makes molecular profiling of solid tumors via mass spectrometry (MS)-based proteomics a daunting task…

Read more: https://www.ncbi.nlm.nih.gov/pubmed/29344855

Posted by Chris Lesher on July 18, 2018

Filed Under: Publications Tagged With: Blonder J, Johann DJ Jr, Prieto DA, Whitely G

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